Ciba Foundation Symposium - Paper Electrophoresis by G.E.W.; & Millar, Elaine C.P. (Editors) Wolstenholme

By G.E.W.; & Millar, Elaine C.P. (Editors) Wolstenholme

Content:
Chapter 1 Chairman's beginning comments (page 1): A. J. P Martin
Chapter 2 normal equipment of Paper Electrophoresis with Examples of its Use in scientific and Biochemical difficulties (pages 2–21): W. Grassmann
Chapter three brief Communications: program of Paper Electrophoresis to the Differential prognosis of canines illnesses (pages 22–29): J. De Wael
Chapter four review of the Albumin?Globulin Ratio of Blood Plasma or Serum through Paper Electrophoresis (pages 30–42): E. M. Abdel?Wahab, V.H. Rees and D. J. R. Laurence
Chapter five research of Human Haemoglobins by means of Paper Electrophoresis (pages 43–57): J. C. White, G.H. Beaven and M. Ellis
Chapter 6 A Paper Electrophoretic learn of the impact of ACTH and Cortisone at the Protein?Bound Serum Polysaccharides in a few sufferers with Rheumatoid Arthritis, Ulcerative Colitis, and so forth. (pages 58–78): Helge Laurell
Chapter 7 Paper Electrophoresis (pages 79–85): E. Koiw
Chapter eight Physicochemical features and their courting to the layout of kit (pages 86–104): Harry Svensson
Chapter nine brief Communications: The mixed impact of Evaporation and Diffusion at the Separation of Serum Proteins by way of Paper Electrophoresis (pages 105–131): J. De Wael
Chapter 10 research of Separated fabrics, I (pages 132–148): E. M. Crook
Chapter eleven brief Communications: quarter below Peaks: shedding Perpendiculars as opposed to Extending Curves to Baseline (pages 149–150): Hugh J. Mcdonald
Chapter 12 a few useful issues in regards to the studying of the Paper Electrophoretic Strip (pages 151–159): S. Chr. Sommerfelt
Chapter thirteen research of Separated fabrics, II (pages 160–171): N.H. Martin
Chapter 14 brief Communications: Protein?Dye Interactions thought of with regards to the Estimation of Protein in Paper Electrophoresis (pages 172–182): Geoffrey Franglen
Chapter 15 a brand new method of the Staining of Lipoproteins (pages 183–196): Hugh J. Mcdonald
Chapter sixteen the way forward for the process in its program to scientific examine and regimen research (pages 197–205): E. L. Durrum
Chapter 17 brief Communications: excessive Voltage Paper Electrophoresis (pages 206–220): B. Kickhofen

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FILDES, J. , and LAURENCE, D. J. R. (1954). J. clin. , 7, 336. REES,V. , and LAURENCE, D. J. R. (1955). Clin.. Chemist, 1, 329. SonmmRFErr, S. C. (1953). Scand. J . din. Lab. , 5, 299. WOLVIUS, D. (1955). Rec. Trav. chim. Pays-Bas, 74, 597. DISCUSSION E’ranglen: I was very interested to hear that Dr. Laurence was using lissamine green, and I think he has been lucky with his particular sample. We have examined 5 different samples which were described as lissamine green by the makers; 3 of them were fairly similar in constitution, but 2 samples from the same manufactuter were quite different.

In the cathode compartment the red colour developed sinks to the bottom, and that makes one suspect that a labyrinth system is perhaps as effective as any in preventing electrode products from entering the zone of interest; particularly, when backflow is employed as in continuous electrophoresis. There is one other point: Prof. Grassmann pointed out that when continuous electrophoresis is carried out using tabs a t the bottom of the curtain only, one gets non-uniform fields. This is true, and the only justification for doing this is that it is a convenient method for keeping electrode products out of the curtain, and thus preventing p H and other changes in the curtain.

And Whitehead, R. (1941). J . Path. , 53, 279), who found that with the Sudan dyes good results could be obtained only when crude mixtures of different isomers of the dye were used; the purified preparations stained rather poorly. A possible explanation for this is that if different isomers are present they will each dissolve in the lipid independently so that a greater amount of dye can dissolve in the lipid with a mixture than with a pure, single component. de Wael: You can find methods in the histological literature in which you must use several different dyes together.

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